Optimization of DCR1 and DCR2 epigenetic annealing temperatures for breast cancer biomarker using in-silico and in-vitro study

The epigenetics of methylated and unmethylated DCR1 DCR2 (decoy receptors 1 2) are genes encoding membrane that can bind to TRAIL causing inhibition in the apoptotic pathway. Epigenetic detection was developed as a biomarker breast cancer. One methods is using PCR. most important step PCR process determination annealing temperature. This research performs Tm analysis insilico program from Neb, insilico, Thermofisher, Promega vitro optimization. Methylated be amplified at temperatures 51.4°C, 52.4°C, 53.6°C, 54.7°C measuring about 600bp according promega. could also 50,1, 49, 48.8 but primers were non-specific sites. 48.8°C, 49°C 50.1°C specific size 500 bp Unmethylated not temperature which analyzed promega, thermofisher.